We use loop resolution Hi-C to track the formation of loop domains across the genome as cohesin is reintroduced. (Top Left) Contact map for 6hr auxin treated RAD21-mAC cells. (Top Right) Contact map for RAD21-mAC cells with 6hr auxin treatment + 20 minute auxin withdrawal. (Middle Left) Contact map for RAD21-mAC cells with 6hr auxin treatment + 40 minute auxin withdrawal. (Middle Right) Contact map for RAD21-mAC cells with 6hr auxin treatment + 60 minute auxin withdrawal. (Bottom Left) Contact map for RAD21-mAC cells with 6hr auxin treatment + 180 minute auxin withdrawal. (Bottom Right) Contact map for untreated RAD21-mAC cells.
See our paper at Cell!
Suhas S.P. Rao, Su-Chen Huang, Brian Glenn St Hilaire, Jesse M. Engreitz, Elizabeth M. Perez, Kyong-Rim Kieffer-Kwon, Adrian L. Sanborn, Sarah E. Johnstone, Gavin D. Bascom, Ivan D. Bochkov, Xingfan Huang, Muhammad S. Shamim, Jaeweon Shin, Douglass Turner, Ziyi Ye, Arina D. Omer, James T. Robinson, Tamar Schlick, Bradley E. Bernstein, Rafael Casellas, Eric S. Lander, Erez Lieberman Aiden. (2017). Cohesin Loss Eliminates All Loop Domains. Cell 171, 305-320.
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